A One-step immunosorbent ELISA method is used in this assay. Samples containing CHOK1 HCP simultaneously react with HRP-labeled goat anti-CHOK1 antibody and anti-CHOK1 antibody coated on the ELISA plate, finally forming a sandwich complex of solid-phase antibody-HCP-labeled antibody. Unbound antigen-antibody can be removed by washing the ELISA plate. The TMB substrate is added to the well for sufficient reaction. The color development is stopped after adding the stop solution, and the OD or absorbance value of the reaction solution at 450/650nm is read with a microplate reader. The OD value or absorbance value is proportional to the HCP content in the solution. From this, the HCP concentration in the solution can be calculated according to the standard curve.
Product HighLights
1. Specific: to CHO K1 cell line
2. Good performance: linear range 3-100 ng/mL, LOD as low as 1 ng/mL
3. Total Experiment time: 2 hours
4. Consistency: CV <10% within batch and <15% between batches
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